| 1. | The membrane was cross linked by 0 . 25 % glutaraldehyde or by dry heat . 2 . investigation of cell compatibility of collagen - chitosan spongy membrane
同时制备未经交联的和干热交联的胶原一壳聚糖多孔膜。 |
| 2. | The practicability and reliability of collagen - chitosan spongy membrane are assessed as a dermal substitute to facilitate the development of tissue engineering skin in future . methods 1
材料和方法一、胶原一壳聚糖多孔膜的制作：以牛健为原料制取二型胶原。 |
| 3. | The density of cultivated dermal fibroblast was adjusted to 5x 105 / cm2 , cell suspension was inoculated on glutaraldehyde cross - linked collagen chitosan spongy membrane to make a cell - matrix complex
真皮成纤维细胞在胶原一壳聚糖多孔膜上的培养：将培养的真皮成纤维细胞浓度调整到5x10 ’冰。 |
| 4. | Investigation of tissue compatibility of collagen - chitosan spongy membrane . ( 1 ) subcutaneous embedding of collagen - chitosan spongy membrane on rabbit . twelve healthy rabbits were divided into four groups randomly
胶原一壳聚糖多孔膜的动物皮下埋植实验：以健康家兔12只作为实验动物，随机分成4组，每组3只。 |
| 5. | ( l ) . cultivation of dermal fibroblast : dermal fibroblasts were harvested by using enzyme digest technique from child foreskin after circumcision . 3 - 4 generation of cells were used as work cells . ( 2 ) cultivation of dermal fibroblast on collagen - chitosan spongy membrane
真皮成纤维细胞的分离培养：取包皮环切术后的幼儿包皮酶消化法分离培养真皮成纤维细胞并传代，以3 4代细胞作为工作细胞。 |
| 6. | We use collagen and chitosan to make spongy membrane as a dermal substitute in order to develop tissue engineering skin . it is very necessary to investigate the biocompatibility and biodegradation of the couagen - chitosan spongy membrane as an assessment of safety and standardization of a tissue engineering medical product
我们采用胶原和壳聚糖制成的多孔膜作为真皮支架来研制组织工程化皮肤，对胶原一壳聚糖多孔膜进行生物相容性研究和降解研究十分必要，这也是组织工程化产品安全性评价和标准化的需要。 |
